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Pakistan Journal of Nutrition

Year: 2017 | Volume: 16 | Issue: 1 | Page No.: 22-27
DOI: 10.3923/pjn.2017.22.27
Effects of Bacterial Inoculants and Enzymes on the Fermentation, Aerobic Stability and in vitro Organic Matter Digestibility Characteristics of Sunflower Silages
Mehmet Levent Ozduven, Fisun Koc and Veysel Akay

Abstract: Objective: This study was carried out to determine the effects of lactic acid bacteria inoculant, enzymes and lactic acid bacteria inoculant+enzymes mixture on the fermentation, cell wall content, aerobic stability and in vitro organic matter digestibility characteristics of sunflower silages. Methodology: Sunflower was harvested at the milk stage of maturity. The treatments were as follows: (1) Control (no additive), (2) Inoculation of lactic acid bacteria (LAB, 2 g t–1, a mixture of Lactobacillus plantarum and Enterococcus faecium applied at a rate of 6.00 log10 CFU LAB g–1 of fresh material) (3) Enzyme (E, 150000 CMCU kg–1 for cellulase and 200000 SKB kg–1 for amylase) and (4) LAB+enzyme mixture (LAB+E, 2 g t–1 a mixture of Lactobacillus plantarum bacterium (6.00 log10 CFU g–1) and 150000 CMCU kg–1 for cellulase and 200000 SKB kg–1 for amylase). After treatment, the chopped sunflower was ensiled in 1.0-l special anaerobic jars, equipped with a lid enabling gas release only. The jars were stored at 25±2°C under laboratory conditions. Three jars from each group were sampled for chemical and microbiological analysis for 2, 4, 8 and 60 days after ensiling. At the end of the ensiling period all silages were subjected to an aerobic stability test for 5 days. Results: In addition, in vitro organic matter digestibilities of these silages were determined. Both inoculants (LAB and LAB+E) increased characteristics of fermentation but impaired aerobic stability of sunflower silages. Lactic acid bacteria+enzymes mixture inoculants decreased neutral and acid detergent fiber content and than control silages. Conclusion: In vitro organic matter digestibility was numerically increased for treated than control silages.

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How to cite this article
Mehmet Levent Ozduven, Fisun Koc and Veysel Akay, 2017. Effects of Bacterial Inoculants and Enzymes on the Fermentation, Aerobic Stability and in vitro Organic Matter Digestibility Characteristics of Sunflower Silages. Pakistan Journal of Nutrition, 16: 22-27.

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