Abstract: Shrimp paste (belacan) has been used as a condiment in many Malaysian cooking. This study aimed to determine the mutagenicity of aqueous extract of shrimp paste from three different places in Pulau Pinang, Malaysia (Balik Pulau, Juru and Pulau Aman) by using Ames test. The test was performed using Salmonella typhimurium strain, TA98 and TA100, in presence and absence of S9 metabolic activation system. Five concentrations of the samples tested were 50, 25, 12.5, 6.25 and 3.125 mg/ml. Macronutrients and mineral content of shrimp paste tested were complied with the Malaysian food act 1983 and Recommended Nutrient Intake (RNI) 2005. Ames test result of shrimp paste from all three places showed number of revertant colonies did not exceed the value of double-fold of negative control either in presence and absence of S9. No significant difference (p>0.05) was found for each concentration tested on the number of revertant colonies between TA 98 and TA 100.