Abstract: Biochemical analysis of non-conventional plant sources, Bermuda Grass, Nursery Grass (sages), Typha and Maize Spike (without grains) was done to determine the nutritional value to replace conventional sources such as rice bran, wheat bran and rice polish to prepare low cost fish feed. Analysis was carried out in the research lab of Institute of Biochemistry, University of Sindh, Jamshoro. It was observed that Bermuda grass and Nursery grass (sagas) have good protein value as 2.84 and 10.97, respectively. It is also observed that these non-conventional plants are a good source of energy and easily available around the year.