Abstract: A study to produce good quality of probiotic supplement based on agriculture industrial waste was done. The study was conducted in three laboratories, namely Feed Technology Laboratory, Animal Feed Science Laboratory and Microbiology Laboratory, Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang. The processing of probiotic manufacture was done using Utama’s et al. (2009) method. Fermentation of agriculture industrial waste process was become the next step after then. The evaluated parameters were the proximate components, total lactic acid bacteria, total Saccaromyces cerevise, gram positive/negative; Salmonella and Coliform according to the Atlas method (2005). The results showed that the treatment of the type of feed ingredients and the incubation period showed the significantly effect (p>0.05) and the components have interaction with proximate parameters such as moisture content, ash content, crude fat, crude protein, crude fiber and nitrogen free extract material. Furthermore, microbiological treatment suggests that career probiotics containing Lactobacillus sp., Saccaromyces cereviseae and Rhizopus sp. On the other hand, determination of E. coli and Salmonella have negative result. Based on the results of this study concluded that the best treatment on a polard carrier with 4 days incubation period has crude protein content of 16.09% and total lactic acid bacteria 3 x 104.