Abstract: Sedentary lifestyle in children caused low physical activity and increase incident of overweight and obesity. Low physical activity leads to physical fitness decline. The objective of this study was to analyze differences of physical fitness in normal and overweight elementary school children. This study was conducted on 108 children as subjects. They were on the fifth grade of elementary school, consisted of normal and overweight student. Physical fitness was measured by some physical tests, i.e., 40 meters sprint, pull up, sit up, jump up and 600 meters run. The result showed that physical fitness of normal and overweight children were significantly different (p<0.05). The average physical fitness score of normal children (13.4±2.1) was higher than overweight children (10.9±1.7). The speed, muscle endurance, explosive muscle power and cardiovascular endurance of normal children were significantly different from overweight children (p<0.05). Nutritional status significantly influenced physical fitness of elementary school children (p<0.05).