Abstract: Temulawak has a role as antioxidant to prevent oxidative stress. This study aimed to assess the effect of temulawak extract on the MDA level (Malondialdehyde) of athletes. The double blind control trials were used, conducted on thirty five football athletes aged 14-18 years of Student Education and Exercise Center. The students were grouped and each received one of five different treatments for 21 days as follows: capsules of temulawak extract which contain of (1) curcumin of 250 mg (2) curcumin of 500 mg (3) curcumin of 750 mg, (4) capsule of multivitamin and mineral (which contain 5000 IU of beta carotene, 200 IU of vitamin E, 500 mg of vitamin C, Zn of 15 mg, selenium of 50 mcg) and (5) placebo, which received a capsule contain of cellulose (avizel). The characteristics of samples of five treatments were not significantly different (p>0.05). Before intervention were no significantly different on mean of MDA and SOD in five treatment groups (p>0.05). After intervention, the MDA level in all groups were decreased, except in placebo group. ANOVA test showed that there were mean differences of MDA level on five groups of interventions (p<0.05). ANCOVA test showed that mean reduction of MDA level on placebo group were significantly different with group received capsules which contain curcumin of 250, 500 and 750 mg/day and MVM (p<0.05). Temulawak extract had capability to reduce MDA level, significantly. The highest reduction of MDA level was done by administration of temulawak extract which contain curcumin 750 mg.