Abstract: Malnutrition and growth failure are prevalent among Persons with Disabilities (PWD) and most trainers or care givers in the Community-based Rehabilitation (CBR) centres had limited knowledge in nutrition management for this vulnerable population. The purpose of this study was to develop and examine the validity and reliability of an evaluation instrument, Nutrition Knowledge, Attitude and Practice Questionnaire in Persons with Disabilities (KAP-nOKU) among trainers in CBR centres, Malaysia. KAP-nOKU was a guided self-administered questionnaire and consisted of 30 knowledge, 15 attitude and 15 practice items. After items generation, it was assessed the content validity by an expert panel; and face validity by nine care givers for spastic children. The revised questionnaire was further pre-tested. This pilot study was a cross-sectional survey and recruited 165 trainers from CBR centres in Perlis, Pulau Pinang and Perak, Malaysia. Item analysis or construct validity, internal consistency and test-retest reliability of KAP-nOKU were examined. Five sub-domains were constructed for knowledge domain and three factor-solutions emerged for attitude and practice domains, respectively. Items with low factor loading and correlation were removed. Internal consistency was good-to-excellent for knowledge (KR20 = 0.63), attitude (CA = 0.67) and practice (CA = 0.82) domains. Test-retest reliability- the intraclass correlation coefficients for knowledge, attitude and practice domains were 0.59, 0.73 and 0.91 (p<0.05). The finalized KAP-nOKU after analysis contained 57 items-29 knowledge, 13 attitude and 15 practice items. KAP-nOKU was an adequately valid and reliable questionnaire which can serve as an assessment tool to evaluate the effectiveness of nutrition education among trainers or care givers in rehabilitation centres, Malaysia.