Abstract: A field experiment was conducted at Elbagair area, Al Jazirah state, central Sudan for two successive seasons 2009/2010-2010/2011. In these experiments pigeon pea plants were either inoculated with five introduced or locally isolated Rhizobium or bradyrhizobium strains each alone or with Bacillus Megatherium var. Phosphaticum strain (BMP) alone and combination of each rhizobial strain with Bacillus Megatherium var. Phosphaticum strain (BMP). In addition to control for comparison. Inoculation with Rhizobium significantly (p<0.05) increased pigeon pea seed yield, 100-seed weight, cookability, moisture content, ash, fat, fiber and protein. BMP inoculation significantly (p<0.05) increased seed yield, 100-seed weight, cookability, fat, fiber and protein content. Rhizobium and BMP co-inoculation significantly (p<0.05) increased seed yield, 100-seed weight, cookability, moisture, fat, fiber and protein content. Inoculation with Rhizobium strain TAL 1132 and co-inoculation with Rhizobium strain USDA 3472 and BMP as phosphobacterin was efficient to give significant yield, with good quality.