Abstract: Thirty four isolates of endophytic fungus produce phytases were isolated from leaf, stem and root fragments of soybean. Two isolates were the best of phytases enzyme producer and identified as Rhizoctonia sp. and Fusarium verticillioides. The phytase production was induced by phytate in medium used. The crude preparations were used in subsequent characterization studies, pH and temperature optimum and compared to other phytases tested and is thus a promising candidate for animal feed applications. The results showed that optimal production of phytase from Rhizoctonia sp. were pH 4.0 and temperature 50oC and pH 5.0, temperature 50oC for Fusarium verticillioides.