Abstract: Bacteriological and physico-chemical quality changes in wheaten white bread flour made for Nigerian market were investigated during storage at room temperature for four months. During storage, bacterial count decreased; between day 15 and day 105, count decreases from 45.0 X 103 cfu/g to 1.0 X 103 cfu/g for flour brand 1 and between day 60 and day 105, count decreases 12.5 X 103 cfu/g to 3.5 X 103 cfu/g for flour brand 3. Statistically, bacterial counts in the different brands of flour during storage show a significant difference. Total coliform count in flour brand 1 decreases d from 4.60 MPN/g (day 15) to zero (day 105) in storage. Significant count in coliform count was obtained for flour brand 1 and flour brand 2 but no significant difference was observed for flour brand 3 and 4 during storage. Staphylococcus albus, Klebseilla pneumoneae and Bacillus subtilis were detected and isolated. Lower pH of below pH 6.0 were recorded at day 105 for flour brands 1, 2 and 4 and the ash content of the various brands of flour was above 0.65% recommended for Nigerian flour with effect from day 90 of storage. Protein, gluten, fat, moisture, and carbohydrate contents were within the acceptable limit values for Nigerian flour.