Abstract: The effects of Pectinex Ultra SP-L concentration, Amylase AG XXL concentration, incubation temperature, pH and incubation time on juice yield, turbidity and viscosity of cloudy passion fruit juice were studied. Enzyme-treated passion fruit pulp sample showed increase in juice yield and turbidity with reduction in viscosity. Response Surface Methodology (RSM) was employed to optimize the hydrolysis conditions for production of cloudy passion fruit juice using enzymatic hydrolysis. The coefficient of determination (R2 values) for juice yield, turbidity and viscosity were greater than 0.900. Statistical analysis showed that Pectinex ultra SP-L concentration, Amylase AG XXL concentration, incubation temperature, pH and incubation time had effect at linear, square and interactive level on yield, turbidity and viscosity. Under the optimum conditions, juice yield extracted from enzyme treated passion fruit pulp was 95.02%, with turbidity 724 NTU and viscosity 1.84cP corresponding to the increase in yield and turbidity by 23% and 31.63% respectively with 0.56 cP decrease in viscosity.