Abstract: The objective of this study is to measure the effect of substituting different levels of shrimp waste hydrolysate (SWH) from Penaeus merguensis for fish meal (FM) in broiler diet. The broilers were randomly assigned in 4 different levels of SWH (0, 4, 8, and 12%) in experimental diet with a Completely Randomized Design. Each dietary treatment was replicated five times. The result of this experiment indicated that weight gain, feed conversion, and percentage nitrogen retention were affected significantly (P<0.05), and no significant differences were found for feed consumption, percentage carcass, percentage abdomen fat, and digestive organ size (liver, proventriculus, gizzard, cecum, and pancreas). The inclusion of SWH until 8% in broiler diet kept the weight gain and feed conversion stable as well as FM in diet, however the inclusion to 12% decreased weight gain and negative effect in feed conversion. In conclusion, SWH could be included to 8% in broiler`s diet or substitute FM as alternative of animal protein source as much as 40%.