Abstract: Palm oil is considered as plant oil in which two types of cooking oil, palm seed oil and palm oil are derived. Palm oil has almost 50% saturated fatty acid and 50% poly unsaturated fatty acid. It is considered to be useful due to metabolites products such as prostacycline and antithrombois in cardiovascular disease (C.V.D) and variation in lipoprotein. In the present study we examined the effect of 12% palm oil on 30 days old male rats (149.3±10.7 g) for 60 days. The changes of weight and food intake were recorded. The result showed that the mean value of rats weight was increased with energy intake in diet (p< 0.05). The serum levels of cholesterol, and HDL-C were increased significantly (p< 0.05), while the levels of triglyceride and LDL-C were decreased but statistically not significant. In conclusion, using palm oil can be useful for prevent of cardiovascular disease.