Abstract: In this study, the effect of acid rains implementation including pH 2-5 was determined on the matured strawberry fruits` levels of A, E, and C vitamins. The acid rains were implemented on the crops in two ways: spraying on upper soil parts and to roots. Vitamin levels of all strawberries were determined by High Performance Liquid Chromatography (HPLC). It was determined that vitamin levels of plants sprayed with simulated acid rains decreased in respect of pH and time when compared with control. Especially, sprayed roots were more affected in respect of vitamin levels than untreated plants.