Abstract: Total Reflection X-Ray Fluorescence (TXRF) technique was used to determine the elemental concentrations of major, minor and trace elements in sesame seeds, Sesame indicum collected from Ogbomoso, Southwestern, Nigeria. Samples were pulverized, digested, and then, analyzed. Eleven elements (P, K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn and Sr) were detected and their concentrations determined. P, K, and Ca were present at the highest concentrations, 0.17 - 0.23 %, 0.46 - 0.53 % and 0.96 - 1.28 % respectively. The results indicate that sesame seed is a good source of essential and beneficial micronutrients required for healthy growth. The accuracy and precision of the technique were assured by analyzing certified standard reference materials IAEA-V-10 Hay (powder).