Abstract: The research work was conducted to determine the production of mycotoxins from the fungi isolated from wine corks stoppers. When comparing the four fractions of Alternaria alternata obtained by the thin layer chromatography with the standards of tenuazonic acid, alter toxin I, altenuene, alternariol, alternariol monomethyl ether and ten toxin, it can be observed that Alternaria alternata only produces alter toxin I, altenuene and alternariol monomethyl ether. The results showed that the production of citrinin by Penicillium citrinum and of fumonisin B1 by Fusarium moniliforme, due to the fact that the fractions isolated by TLC of these fungi coincide with the controls for such mycotoxins. Fusarium solani did not produce fumosin B1.