Abstract: Background and Objective: Local micro organism (LMO) is the result of the fermentation of various mixtures of organic matter. One of the organic materials used, based on the local wisdom of West Sumatra, is tapai (fermented Cassava), which is used as a bio activator in the manufacture of organic fertilizer. The research aims to produce organic fertilizers that meet national quality standards in terms of the physical and chemical quality of fertilizers as well as to determine the diversity of bacteria in bio activators through next-generation sequencing analysis. Materials and Methods: The organic ingredients for bio activators, cow feces as basic fertilizer ingredients, materials for analyzing bacterial diversity, LMO gDNA was extracted using ZymoBIOMICS DNA Miniprep Kit DNA and sequenced using Oxford Nanopore Technology. Results: On a scale of 1-3, the physical quality of organic fertilizers had an average value of 2.58 for smell, 2.83 for texture and 2.58 for color. The chemical quality of organic fertilizers is C-organic (23.56%), nitrogen (1.60%), carbon and nitrogen ratio (14.75%), phosphate (0.47%) and potassium (0.64%). The results of the analysis of bacteria on the bioactivator consisted of 7 phyla, 9 families, 45 genres and 297 species. The most common species is Lentilactobacillus hilgardii (62%). Conclusion: The organic fertilizer produced using the mole tapai bio activator complies with Indonesian national standard 19-7030-2004 based on physical and chemical parameters. The type of bacteria that dominates the bioactivator is the lactic acid bacteria group, which reaches 90%.