Abstract: Background and Objective: The emergence of antibiotic resistance is a primary global health concern. As a result, there is an urgent need for new strategies to combat antibiotic-resistant bacteria. One of these essential strategies is the combination of medicinal plants and antibiotics as an alternative to using antibiotics alone which was the objective of this article. Materials and Methods: Nine plant materials were collected from different Egypt localities and then extracted by water. Water extracts were filtered and added with Mueller-Hinton agar during preparation. Nine test bacteria and 13 standard antibiotics were used in the disc diffusion sensitivity method. Results: The activity of Amikacin was increased when combined with most different plant extracts against Escherichia coli while antagonistic against Pseudomonas aeruginosa. Aztreonam, Ceftriaxone, Gentamicin and Nalidixic acid antibiotics showed antagonistic or indifferent effects when combined with most different plant extracts against E. coli, Klebsiella pneumonia and P. aeruginosa. The synergistic effect was achieved in Aztreonam when combined with all plant extracts, while Nalidixic acid showed antagonistic when combined with most plant extracts against Proteus mirabilis. The antagonistic effect was achieved in Aztreonam, Ceftriaxone and Nalidixic acid when combined with Achillea fragrantissima, Artemisia monosperma and Leptadenia pyrotechnica, also Aztreonam with Lycium shawii extract against Salmonella typhimurium. The A. fragrantissima and A. monosperma increase the activity of Novobiocin and Vancomycin against Bacillus cereus and Ampicillin and Cefazolin against Staphylococcus aureus but Novobiocin activity increased with most plant extracts against S. aureus. Conclusion: The combinations of antibiotics with the extracts of medicinal plants displayed varying degrees of effects, synergistic, antagonistic and indifferent according to antibiotic type, plant extract and test organism.