Abstract: Background and Objectives: Anthraquinone synthetic dyes are widely used in textile, dyeing and paper painting. The discharge of these dyes into the environment causes detriment. The removal of physiochemical dyes is sometimes unsuccessful and expensive. Biological removal is inexpensive, eco-friendly and may break down organic contaminants. In the current work, a fungal technique was applied to decolorize and detoxify dye. Materials and Methods: Dye decolorizing fungi isolation, selection and identification of the most effective isolate and dye decolorization optimization based on carbon and nitrogen sources. In addition, the product's cytotoxicity and metabolites were tested. The enzyme activities were measured to determine dye decolorization. Results: Decolorization of reactive blue 19 dye by the most effective fungal strain isolate (5BF) isolated from industrial effluents were studied. This isolate was identified as Aspergillus flavus based on phenotypic characteristics and confirmed using 18S rRNA gene sequencing. Thin-layer chromatography indicated that this strain is aflatoxins free. Furthermore, metabolites produced from dye treatment with A. flavus were assessed using gas chromatography-mass spectrometry. Toxicity data revealed that A. flavus metabolites were not toxic to plants. Using a one-factor-at-a-time optimization technique, a maximum decolorization percentage (99%) was obtained after 72 hrs in the presence of mannitol and NH4NO3 or NH4Cl as carbon and nitrogen sources. Two enzymes (laccase and manganese peroxidase) were shown to be active during dye decolorization by A. flavus. Conclusion: The A. flavus strain was shown to be safe when it came to removing dye from a synthetic medium with high efficiency and their metabolites had no negative influence on the environment. As a result, this strain will be used in the future for dye wastewater bioremediation.