Abstract: Background and Objective: The possibilities of using J. curcas seeds oil as bio-fuel in Nigeria requires large scale production of the plant which can be achieved through plant tissue culture technique. This study aimed to determine the effects of varying concentrations of silver nitrate (AgNO3) solution on the regeneration of J. curcas from cotyledonary petiole explants. Materials and Methods: The experimental layout followed completely randomized design with 20 replications for factors such as varying concentrations of silver nitrate, soaking period and orientation of the explants on MS-media. Data were analyzed using analysis of variance and means were separated using Duncan Multiple Range Test at p<0.05. Results: Application of AgNO3 solution at 10 mg L–1 promoted greater percentage shoot buds regeneration and number of shoot buds per explant. Twenty minutes process-time favoured higher shoot buds regeneration and placement of explants in slant position was found to be conducive orientation for regeneration of shoot buds. L-arginine between 7 and 10 mg L–1 promoted elongation of regenerated shoot buds. Rooting of the elongated shoot buds was faster in culture receiving 20 mg L–1 of indole-3-butyric acid (IBA). Regeneration of shoot buds regeneration was better in in vitro than in in vivo. It took 126-135 to achieve regeneration of plantlets. Conclusion: Recovery of plantlets was better when in vitro cotyledonary petiole explants were treated with 10 mg L–1 of AgNO3 solution, soaked for 20 min and placed in a slanted position on MS media and receiving further treatments of 7-10 mg L–1 of L-arginine and 20 mg L–1 of IBA.