Abstract: Background and Objective: Soybean crop occupies a high position among cereal crops because it is a highly nutritious plant. This investigation aimed to take advantage of the imported soybean lines in improving the local varieties. Materials and Methods: Three local varieties and 4 imported lines of soybean plant were planted during two seasons for estimating some genetic parameters including (Heritability in broad sense, PCV (%), GCV (%), DZ, expected genetic advance and genetic advance as percentage of mean) in some agro-morphological traits besides molecular characterization for the seven soybean materials using 6 SCoT primers. Results: The four imported soybean genotypes besides the local cultivar Giza 111 exhibited highly trend for all studied traits in both seasons. Heritability and genetic advance were exhibited significance and high values in most studied traits under both seasons. Conclusion: The imported soybean genotypes confirmed significant excellence in all studied traits and recorded highly genetic stability for both seasons depending on results obtained of all genetic parameters. Therefore, the decision to use it as a new and good genetic source to improve, develop and raise the productivity of Egyptian soybean varieties as well as its resistance to biotic and abiotic stresses will be right and fruitful.