Abstract: Background and Objective: Jatropha variegata is traditionally used in Yemeni folk medicine for antiseptic and hemostatic purposes. In this study, the methanolic extract of the plant leaves was evaluated for its antioxidant, antibacterial and wound healing activity. Materials and Methods: The antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. The antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was tested using disc diffusion and broth microdilution assays. In vivo, the ability of the extract to accelerate wound healing in rats was evaluated using both wound area measurements and histological analyses. Results: The leaves extract exhibited strong antioxidant activity with an IC50 value of 16.7 μg mL–1. The extract exhibited moderate antibacterial activity against S. aureus with inhibition zones of 10.6 mm, and the Minimum Inhibitory Concentration (MIC) value was 5 mg mL–1. The extract significantly accelerated the rate of wound healing closure compared to those treated with the vehicle. In addition, histopathological analyses of wound granulation tissues showed significantly better healing signs after 14 days in the extract-treated groups, with denser collagen deposition at the injury site. Conclusion: The leaves of J. variegata appear to contain bioactive compounds that may be utilized clinically in combating oxidative stress and in wound management.