Abstract: Background and Objective: Study on medicinal plant extract is gradually interested and distributed, especially their biological activities. The present study aimed to determine the enzyme inhibition and antimicrobial activities of the fractionated extracts of wild grape (Ampelocissus martinii Planch.) seeds. Materials and Methods: Wild grape seeds in different growth stages were extracted with methanol before fractionation by silica gel chromatography. The anti-glucosidase and anti-tyrosinase enzyme activities of the extracts were then tested by using UV-Vis spectrophotometry and antimicrobial activities were observed from MIC, MBC values and time killing assay. Results: The sub-fraction of immature stage eluted by ethyl acetate/methanol at 75/25 (%v/v) has the highest enzyme inhibition activity and the most potent efficiency for time kills profiles. The MIC values of the potent immature, mature and ripe fractioned extracts were ranging from 1.25-50.00, 1.25-50.00 and 1.56-25.00 mg mL–1, respectively, while the MBC values ranged from 3.12-6.25, 3.12-25.00 and 3.12-25.00 mg mL–1, respectively. Conclusion: The wild grape seed composed of α-glucosidase and tyrosinase inhibition and antibacterial activities compounds. The wild grape seed extracts may be used as active ingredients sources of health-supporting products or cosmetics.