Abstract: Background and Objective: Malaria is still a severe health problem especially in developing countries which occur and young children are the most affected. The present study was designed to compare the insecticidal potential of Lantana camara leaves extract alone and its synthesized nanoparticles against Anopheles multicolor. Materials and Methods: Copper nanoparticles CuNPs were synthesized by green bio-reduction method using aqueous extract of leaves of Lantana camara plant. The CuNPs formation was confirmed by ultraviolet visible spectrophotometer (UV-VIS) and Transitional Electron Microscopy (TEM). The application of L. camara extract and its synthesized CuNPs on different stages of A. multicolor were adopted. Results: The biosynthesized CuNPs were spherical with the average sizes of 11-17.8 nm. The highest insecticidal effect (100% of larval mortality) achieved at high dose (140 ppm) of L. camara leaves extract alone comparing with that occur at low dose (20 ppm) of CuNPs synthesized L. camara leaves. The LC50 and LC90 for the 4 instar larvae were 63.5 and 119.9 ppm for plant extract alone compared to 12.6 and 18.4 ppm for CuNPs preparation. Conclusion: So, this study proved that CuNPs preparation of L. camara leaves is highly efficient compared to the plant extract alone and more economic as less quantities were used. Also, awareness against invasion of Anopheles mosquito vectors with effective preventive measures to protect from malaria infection.