Abstract: Background and Objective: Quality banana fruit is significantly related with number and type of metabolite produce. Metabolite production of Musa acuminata L. is effected by abiotic factors such as salinity. So the purpose of this research was to study the effect of plant response under salinity stress and also identified the chemical compound especially phenolic compound. Materials and Methods: Explants used in this research were shoot of Musa acuminata L., Murashige and Skoog medium, Benzyl amino purine, sugar, agar, sodium chloride. The research was conducted in two stages; stage (1): Developing in vitro culture of shoot of Musa acuminata L. with NaCl treatment i.e., 0, 50, 100, 150 and 200 mM concentration, stage (2): Gas Chromatography Mass Spectrometry (GCMS) analysis on the shoot of in vitro banana plantlets. Results: The findings of this study showed that compound content of shoot of banana plantlets were Amine, Ester, Propane, Keton, Alkohol, Phenol, Methyl, Acid and it was observed that Ester (27.515%) was the highest detected compound. Conclusion: It is concluded that the salinity had affected the plant growth. The analysis showed that the highest compound content of Musa acuminate L. Barangan cultivar was Ester and Phenols.