Abstract: Background and Objective: Breast cancer is the most frequently diagnosed cancer in women. Chemotherapy is the main method of breast cancer treatment but there are side effects. Carica papaya leaves is vegetable foods consumed by most people of Indonesia have potential as anticancer. The aim of this study was to investigate anti-proliferative and apoptotic induced effect of aqueous papaya leaves extracts on human breast cancer cell lines MCF-7. Materials and Methods: Inhibitory on cell proliferation was measured by MTT assay while apoptosis induction was measured using Annexin V. Results: The results showed that papaya leaf can inhibit the proliferation of human breast cancer cells MCF-7 with IC50 in 1319.25 μg mL–1. The IC50 values of papaya leaf extract was higher than the IC50 value quercetin and doxorubicin. Papaya leaf extract can also induce apoptosis of breast cancer cells MCF-7 about 22.54% for concentration 659.63 μg mL–1 and about 20.73% for concentration 329.81 μg mL–1. The percentage of cell apoptosis of papaya leaf extract lower than doxorubicin but higher than quercetin. Conclusion: This study indicated that papaya leaf extract have potential as anticancer through mechanism anti-proliferation and apoptosis induction.