Abstract: Research of fluctuations in Diptera’s larvae in Phytotelmata had been conducted at three locations in West Sumatra, Indonesia; Padang, Bukittinggi and Payakumbuh, which aimed to determine the number and fluctuations Diptera larvae in Phytotelmata. The results obtained; the highest number of individual larvae Diptera in Phytotelmata was 7109 Aedes albopictus larvae (49.56%), followed by larvae of Culex tritaeniorhynchus with 2409 individuals (16.80%). Larvae fluctuated every month and tent to increase in November and December. There was no difference in the number of Diptera larvae individuals inhabiting pandan, taro, and pineapple, but there were significant differences between the three types of Phytotelmata (pandanus, taro and pineapple) with bamboo (p<0.05). Number of individual larvae in Phytotelmata negatively correlated with temperature and rainfall, but positively correlated with humidity (r = 0.44: p<0.05).