Abstract: The effect of exercise and recovery period on adiponectin level is not still cleared. The aim of this study was to evaluate the effect of different intensities of running on serum adiponectin and lipoproteins levels in male rats. In this experimental study, one hundred and sixty rats aged 2 months years old (250±5 g) were randomly assigned into four groups including the control and the 3 groups running at the speeds of 18, 24 and 30 m min-1 for 30 min. Serum adiponectin, Low Density Lipoprotein (LDL) and High Density Lipoprotein (HDL) levels were evaluated in four stages: Before running, immediately, 30 min and 5 h after the running finished. In different stages, 10 rats of each group were anesthetized and blood were collected from abdominal aorta. Serum adiponectin concentrations increased immediately after running in the rats ran with the speeds of 18, 24 and 30 m min-1 in 30 min (p<0.05). Thirty minute after running, serum adiponectin concentrations did not change only in the rats ran with the speed of 18 m min-1 (p = 0.46). Five hours after running, serum adiponectin concentrations approximately reached into the before running levels in the rats ran with the speeds of 18, 24 and 30 m min-1 (p<0.05). There was no significant difference in serum LDL and HDL concentrations between and within rat groups (p>0.05). Serum adiponectin concentrations rose when the running intensities in one exercise session increased in male rats. During recovery period, serum adiponectin concentrations decreased with the same pattern in different exercise intensities.