Abstract: Selected environmental samples such as water, fishes (Tilapia mariae and Clarias gariepinus), earthworm (Libyodrillus violaceous) and soils were obtained from the Otokutu end of Warri River, digested with acid mixtures and analyzed for trace metal concentrations using the atomic absorption spectrophotometry. The trace metals measured include; zinc, lead, copper, arsenic, iron, cadmium and mercury. The results obtained showed variations in the concentrations of metals in the entire samples analyzed. Lowest metal concentrations were recorded in the water samples. Trace metal concentrations in Tilapia mariae, Clarias gariepinus and Libyodrillus violaceous were higher than levels recorded in water and soil samples, respectively. The elevated concentrations of lead, arsenic, iron, cadmium and mercury were traceable to anthropogenic wastes and activities of industries operating in Warri and its environs.