Abstract: Tridax procumbens L., Asteraceae, has been extensively used for various ailments in the Ayurvedic system of medicine. Previous studies have revealed remarkable phytoconstituents from Tridax procumbens L. with significant antioxidant activity. The aim of the present study is to measure the anti-DPPH activity of the purified isolated compounds from n butanol soluble part and ethyl acetate soluble part of successive methanolic extract of Tridax procumbens L. We thus quantified the total phenolic and total flavonoids in different purified isolated compounds, the whole of the tests were evaluated with a sample conc. of 100 μg mL-1 and were determined spectrophotometrically using Folin-ciocaltue and AlCl3 reagents, respectively. DPPH (1, 1-diphenyl, 2-picryl hydrazyl) assay was used to determine the in vitro antioxidant activity of different isolated compounds. Isolated compounds, one from ethyl acetate soluble part (EF-I) and one from n butanol soluble part (BF-II) were reported to possess a significant anti DPPH activity with lowest IC50 values 67.26 and 80.90 μg mL-1, respectively while comparable to standard ascorbic acid with IC50 value of 59.62 μg mL-1, due to the high concentration of phenols 146.4 μg mL-1 from EF-I and 142.2 μg mL-1 from BF-II and flavonoids 48 and 42.5 μg mL-1 found in EF-I and BF-II isolated compounds, respectively.