Abstract: In recent decades, there has been a drastic increase in the incidence and prevalence of diabetic mellitus. The aim of this study was to evaluate the in vitro inhibitory effect of Azadirachita indica leaf extract on the activity of α-amylase and α-glucosidase as a means of alleviating hyperglycemia and managing diabetes mellitus. Aqueous extract of Azadirachita indica exhibited most potent α-amylase inhibitory activity with IC50 value of 9.15 mg mL-1 and acetone extract exhibited most potent α-glucosidase inhibitory activity with IC50 value of 5.00 mg mL-1. Kinetic studies revealed both acetone and aqueous extract to exhibit mixed non-competitive inhibition for α-amylase and α-glucosidase. It can be concluded that the antidiabetic potential of Azadirachta indica may be due to its ability to inhibit both α-amylase and α-glucosidase. The presence of phytochemicals such as flavonoids, tannins and saponins in this plant may be responsible for its inhibitory activity on the two enzymes studied.