Abstract: Date fruit juice contains high concentration of simple sugars ranging from 65 to 75% (w/w) in dry form. In this study, the potential of date fruit juice as biobutanol fermentation medium by C. acetobutylicum was investigated. The fermentation process was carried out at initial pH of 5, 6 and 7, incubation temperature of 30, 35 and 40°C for 72 hours. The date fruit concentrations tested were 10, 20, 30 and 40 g L-1. Medium containing 30 g L-1 of date fruit at 35°C incubation temperature with initial medium pH 7.0 gave the highest concentration of solvents of 3.1, 0.1 and 1.1 g L-1 butanol, ethanol and acetone respectively. The yield and productivity of biobutanol were 0.32 g g-1 and 0.044 g L-1/h respectively, while for total ABE were 0.45 g g-1 and 0.06 g L-1 h, respectively.