Abstract: The adverse effects of sea water salinity on number of nodules, nitrogen content, nitroginase activity, Chlorophyll a and b content, proline accumulation and protein pattern of faba bean plants (Vicia faba commercial cultivar Nubaria 1) were investigated. Faba bean plants were irrigated with sea water at 20, 25, 30, 40 and 50% concentrations and inoculated with rhizobial isolate ARC307 or with gamma rays treated isolates namely; ARC1, ARC2, ARC3, ARC4, ARC5, ARC6 and ARC7. Nodules number, nitrogen content, nitroginase activity and chlorophyll a and b content parameters were decreased by increasing sea water salinity with all used isolates, while proline accumulation parameter increased. At the same time, ARC2 isolate showed the highest values for these parameters above all isolates including the parental isolate ARC307 at all studied concentrations except for proline accumulation parameter, it was the least. Therefore, ARC2 considered as a promising isolate for salt tolerance. Salinity enhanced the occurrence of particular novel proteins in faba bean plants infected with ARC2 isolate.