Abstract: In this study, investigation of some anther structures in Sanguisorba minor Scop. subsp. muricata was aimed. Therefore, anthers were squashed by modified glycerine-gelatine method and additionally different histochemical stainings also were tested. The result illustrated that in different parts of anther, such as marginal, connection tissue, starch and protein granules accumulated. Furthermore, during maturation these deposits were detected in pollen grains also. In addition, a very interesting feature, balloon-like structures filled by proteins, were observed in anther cavity. In the light of these findings, their importance on pollen development was discussed.