Abstract: Jamur Tanduk (Termitomyces eurirrhizus Berk) is one of uncultivated edible mushrooms from genus Termitomyces found in various regions in Indonesia. The purpose of this study is to analyze the chemical structure of its β-glucan compound that possesses bioactivity as lowering cholesterol levels. Isolation and identification of β-glucan compound from water extract of Jamur tanduk (local name), Termitomyces eurirrhizus Berk collected in Sumedang has been done. β-glucan was isolated with Wasterlund methods and obtained as white powder. Isolated compound was identified based on interpretation of spectroscopies data such as Ultra-Violet (UV), Infra-Red (IR) and nuclear magnetic resonance one dimensional such as 1Hidrogen-Nuclear Magnetic Resonance (1H-NMR), 13Carbon- 13C-NMR) and Distortionless Enhancement by Polarization Transfer (DEPT) and two dimensional such as Correlation spectroscopy (COSY), Hetero Multiple Quantum Connectivity (HMQC) and Hetero Multiple Bond Connectivity (HMBC). The determination of molecule weight of sample based on viscosity measurements and obtained that β-glucan has 322 molecules of glucose.