Abstract: Breed and seasonal influence on testicular morphometry, Gonadal Sperm Reserves (GSR), Extragonadal Sperm Reserves (ESR) was investigated in the barred Plymouth rock and the Nigerian indigenous breeds of the domestic fowl. Twenty sexually matured birds per breed were studied in the four seasons of the year. Breed significantly (p<0.01) influenced the body weight (2.11±0.05 vs.1.58±0.02), paired testes weight (21.92±1.46 vs. 12.43±0.91) and all other morphometric parameters with the barred Plymouth Rock showing superiority over the Nigerian indigenous breed. The GSR, ESR and their efficiencies (GSR/g, ESR/g) were highly and significantly (p <0.01) higher in the barred Plymouth rock. Season neither significantly (P>0.05) influenced the testicular morphometric parameters, GSR, ESR nor gonadal sperm reserve/gram testis (GSR/g). The higher numerical differences observed in morphometric parameters, GSR and ESR during the early and late dry seasons suggested that the dry season favors sperm production, hence sperm reserve potentials. The testicular weight was significantly (p<0.001) correlated not only to the body weight (r = 0.63) but to paired Tunica albuginea (= 0.98, p<0.001) and paired epididymal weight (r = 0.69, p<0.001). There was also a significant (p<0.001) and positive correlation between the testicular weight and GSR (r = 0.84, p<0.001). The GSR/g (r = 0.77; p<0.001) ESR (r = 0.86; p<0.001) and ESR/g (r = 0.50, p<0.001). Based on these observations it is concluded that genetic improvement of the Nigerian indigenous breed of the domestic fowl can be carried out by increasing their body weight, hence, testicular weight through crossbreeding with the heavier barred Plymouth rock to achieve improved sperm production and reserve for year round artificial insemination.