Abstract: Investigation was conducted during 2006 and 2007 to detect and determine typing of Cucumber mosaic virus (CMV) in field-collected samples from Razavi Khorasan Province. Leaves showing chlorosis, mosaic, distortion and shoestring symptoms were collected from the Mashhad Regions (Astan-e-ghods and Khaje-rabie), Neishabour, Torbat-e-heidarye, Ghochan, Chenaran, Fariman, Shirvan, Kalat and Kashmar. Samples were transferred to laboratory in order to detect the virus in the collected samples, Samples were tested by DAS-ELISA. Typing was done for those serologically positive-reacted samples by RT-PCR-RFLP. Specific primers have amplified 650 bp fragments of RNA2, in RT-PCR assay. Digestion was performed by restriction enzyme MluI. Present research showed that all of collected samples were in subgroup IA.