Abstract: In this study, ELISA (Enzyme Linked Immunosorbent Assay) technique was used for detection of aflatoxin M1 in UHT milk sold in Bursa-TURKEY for consumption. A total of 50 samples of commercial UHT (Ultra High Temperature) whole milk were analyzed. Aflatoxin M1 residues were detected in all samples (100%) studied in different levels. The mean value was 101.2±53.8 ng L-1. Although, 40 (80%) were below the limit, the remaining 10 (20%) were well above the limit permitted by European Community and Turkish Food Codex. Serious risks for public health exist from milk consumption. Therefore, milk has to be controlled periodically for AFM1 contamination. Also, dairy cow feeds should be stored in such a way that they do not become contaminated.