Abstract: In this study, two field experiments were carried out during the two growing seasons (2005-2006 and 2006-2007) to investigate the role of some applied antioxidants (spermine 10 mg L-1 and ascorbic acid 200 mg L-1) in counteracting the harmful effect of soil salinity stress (10.1 or 14.6 dS m-1) on canola plants. Growth characters, yield and its components as well as biochemical constituents were studied in the two growing seasons. The results showed that all growth characters including; plant height, leaves number and area/plant, shoot and root dry weight as well as yield and its components including; fruit number/plant, number of fruiting branches, seed number/fruit, seed yield/plant and seed oil content of canola plant were decreased with increasing soil salt level (A2) comparing with (A1). On the other hand, applied antioxidants spermine 10 mg L-1 and ascorbic acid 200 mg L-1) increased growth and yield of canola plant during the two growing seasons. However, the applied antioxidants were more effective under the first soil salt condition (A1) soil salt stress levels (A2) decreased each of photosynthetic pigments, K and P contents, while increased proline, soluble sugar, ascorbic acid, Na and Cl contents compared with (A1). On the other hand, applied antioxidants increased each of photosynthetic pigments, proline, soluble sugar, N, K and P contents, while decreased Na and Cl contents in canola plant under soil salt stress (A1 and A2) during the two growing seasons. It could be concluded that applied antioxidants could counteract the harmful effect of salt soil stress on growth, yield and biochemical constituents of canola plant.