Abstract: In this study antioxidant activity of methanol extract of Sambucus ebulus L. flower was investigated employing various in vitro assay systems, i.e., DPPH and nitric oxide radical scavenging, hydrogen peroxide scavenging, reducing power, iron ion chelating power and linoleic acid. IC50 for DPPH radical-scavenging activity was 228±12 μg mL-1. The extract showed very high activity in the reducing power assay that was comparable with positive control, vitamin C. The extract showed good nitric oxide-scavenging activity (IC50 = 309±14 μg mL-1). It was found that antioxidant activity was dose dependent i.e., activity was increased with the increase of their concentrations. The extract showed very weak activity in iron ion chelating (IC50 = 1.3±0.07 mg mL-1). It is showed very good activity in scavenging of hydrogen peroxide. IC50 for scavenging of extract was 59.5±3.3 μg mL-1. The extracts exhibited no activity in linoleic acid model. The total phenolic content of flower was 56.3±2.81 mg gallic acid equivalent g-1 of extract powder and total flavonoid content was 14.5±0.72 mg quercetin equivalent g-1 of extract powder by reference to standard curve.