Abstract: A TWINSPAN classification of 60 sample plots from the Khanikan forest (North of Iran) is presented. Plant types were determined from field observations and sample plot data arranged and analyzed in association tables. The types were defined on the basis of species patterns of presence, absence and coverage values. Vegetation was sampled with randomized-systematic method. Vegetation data including density and cover percentage were estimated quantitatively within each quadrate and using the two-way indicator species analysis. The objectives of the study were to plant type`s classification for Khanikan lowland forest in North of Iran, Identification of indicator species in plant types and increase our understanding in regarding to one of Multivariate analysis methods (TWINSPAN). Five plant types were produced for the study area by TWINSPAN, i.e., Menta aquatica, Oplismenus undulatifolius, Carex grioletia, Viola odarata and Rubus caesius. Therefore, at each step of the process, the program identifies indicator species that show strongly differential distributions between groups and so can severe to distinguish the groups. The final result, incorporating elements of classification can provide a compact and powerful summary of pattern in the data set.