Abstract: The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L-1 BA and 2.0 mg L-1 NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L-1 BA with 0.2 mg L-1 NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L-1 each of IBA and IAA.