Abstract: A total of 200 genomic DNAs were isolated from the four breeds of duck: Muscovy, Pekin, Khaki Campbell and Native, through a modified salting out procedure. The samples were used in a Polymerase Chain Reaction (PCR) with 27 RAPD markers. Amplified PCR-products with the markers were separated on a 2% agarose gel and stained with ethidium bromide. To evaluate the bands, polymorphic and monomorphic bands were described. Genetic similarity and genetic distance were calculated. The RAPD analysis data from 7 primers were utilized in estimating genetic similarity between four breeds, which ranged from 0.46 to 0.77 between the breeds. The maximum genetic distance was observed between Muscovy and Native duck breeds (0.76).