Abstract: The establishment of a micro-propagation protocol for banana (Musa sp.) cv Basrai, was carried out by using meristematic stem cuttings, as an explant. Upto 60% micro-propagation efficiency was increased, when organogenesis (MS; 10.0 μM BA; 15.0 μM IAA) was carried out on medium solidified with 3.60 g L-1 phytagel for 3 weeks, while shoot induction and its multiplication (MS; 10.0 μM BA) were obtained on the medium with 1.0 g L-1 phytagel for 10 days and 2.0 g L-1 phytagel for 20 days, respectively. One culture (20.0 μM BA and 4.0 μM NAA or/and 6.0 μM TDZ) somewhat callus formation was observed but later on was proceeded to death, instead of multiplication. The developed plantlets were cultured on MS basal medium supplemented with IBA (0.50 mg L-1) for root induction. Through this method, complete micro-propagated plantlets were obtained within 3 months.