Abstract: Cotton is an important crop in Turkey. Johnsongrass is one of the worst weeds in cotton fields worldwide. Field experiments were carried out in 1992, 1997 and 2002 to show the effect of johnsongrass densities on cotton yield. Johnsongrass densities were 0, 1, 2, 4, 8, 16 and 32 plants per 8 m and 0, 3, 6, 8, 11, 14 and 22 plants per 4 m of cotton row. Data were fitted to equations Y = 1/(a+b*X8) in 1997 and 2002 for the number of johnsongrass in 8 m of row length and Y = 1/(a+b*X4-1/2) in 1992 and 1997 for the number of johnsongrass in 4 m of row length. Cotton yield was affected by all johnsongrass densities. One johnsongrass plant in 8 m of cotton row decreased cotton yield 4.82 and 9.42% in 1997 and 2002, respectively.