Abstract: The antimicrobial activity of vermiextract was assessed against four human pathogenic microorganisms. Especially strains of Proteus species are a prominent cause of urinary tract infection in children. The present investigation deals with particular urinary tract pathogenic organisms like E. coli, Proteus Providencia Morganella species. Screening was performed by disc diffusion method. The zone of inhibition range from 17 to 19 mm against 1.0X108 cells of each culture at 1 mg concentration of vermiextract. Vermiextract was most effective against Proteus species. The MIC (Minimum Inhibitory Concentration) was estimated by microdilution method.