Abstract: This study describe the reproductive biological characteristics and population parameters of the goby, Pseudapocryptes elongatus (Cuvier, 1816), in the coastal mud flat areas of the Mekong Delta, Vietnam. A total of 1058 specimens was collected from January 2004 to June 2005 and results showed that the breeding season occurred with two spawning peaks in July and October. Length at first maturity (Lm) was 15.4 and 16.3 cm for females and males, respectively. The batch fecundity estimates ranged from 2,652 to 29,406 hydrated oocytes per ovary in the fish ranging from 12.8 to 22.4 cm TL. Length frequency data of the goby ranging from 9.0 to 24.0 cm TL were analyzed using the FiSAT II software. The von Bertalanffy growth parameters were determined as L8 = 25.9 cm, K = 0.66 year-1 and to = - 0.26 year-1. The longevity (tmax) of the goby was estimated to be 4.55 years. There were two recruitment peaks with very different magnitudes and the means of these two peaks were separated by an interval of 5 months. Length at first capture (Lc) was 10.05 cm, the instantaneous fishing mortality rate (F = 1.38 year-1) and natural mortality rate (M = 1.46 year-1) accounted for 49 and 51% of the total mortality (Z = 2.84 year-1), respectively. Relative yield-per-recruit and biomass-per-recruit analyses gave Emax = 0.65, E0.1= 0.55 and E0.5 = 0.33. Results show that the fish stock is subjected to growth overexploitation.