Abstract: RAPD markers variations were studied in eleven olive cultivars. Seventeen RAPD primers out of 27 produced 610 bands in total. Four hundred forty four bands were polymorphic bands (72.70 %) and 166 bands were monomorphic (27.20 %). Primer C05 produced the highest number of bands (56), while primer C03 produced the lowest number of bands (7). Specific bands were observed in some of the cultivars, which may be used in the cultivars discrimination. Different similarity coefficients determined among the cultivars studied, showed the highest value of similarity between cultivars Kroneiki and Manzanila. Different clustering methods showed distinctness of the olive cultivars studied. The grouping of the cultivars did not correlate completely with their place of origin or fruit characteristics.