Abstract: To stimulate the production of taxol (paclitaxel) by cell suspension cultures of Taxus cuspidata var. nana, two kinds of elicitors and a biogenetic precursor were used in F4G4 culture medium. Paclitaxel production was enhanced by each elicitor as well as by the biogenetic precursor and by a combination of the two elicitors or one elicitor and the biogenetic precursor. The amount of paclitaxel produced (16.6 mg L-1) was greatest when the cell suspension cultures were conducted in F4G4 medium containing chito-heptaose (8 mg L-1) and jasmonic acid (21 mg L-1). The productivity was enhanced 4.1 fold compared to the control. The amount of paclitaxel produced was increased by supplying air to the cultures, though the productivity depended on the amount of air supplied. However, no enhancement of production was observed when a combination of air and chito-heptaose was provided to the cell suspension cultures.