Abstract: Many mutagens and carcinogens may act through the generation of Reactive Oxygen Species (ROS) in cells that maybe related to cancer. The carotenoid astaxanthin-the red pigment for the attractive coloration in the skin and flesh of shrimp-has attracted considerable interest in recent years because of its superior antioxidative activity to most of the hydrophobic antioxidants. According to the important role of carotenoids in human health, the main carotenoid pigment in flesh and by-products of the different shrimp species from penaeidae family-Astaxanthin-was extracted and separated by thin layer chromatography using silica gel, subsequently. The identifications were confirmed by fourier transform infrared (ET-IR) spectroscopy. The effect of these extracts on mutagenicity and carcinogenicity induced by Sodium Azide and potassium permanganate was investigated in bacterial assay system, i.e., the Ames test with salmonella typhimurium TA100, TA104. These strains have a certain mutation in their histidin operon, which are related to the external histidin source. Such a mutation can be reversed through contact with a diagnostic mutagen, but in shrimp extracts prevented from effect of this mutagen in which, after repeated test series under standardized condition, the anticarcinogenic effect of flesh and skin of shrimp was proven and the shrimp skin showed stronger anticarcinogenic effect in comparison with shrimp flesh. Different shrimp species from penaeidae family indicated similar antricariongenic effects.